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viral construct  (Addgene inc)


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    Structured Review

    Addgene inc viral construct
    Viral Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+flex+gfp+plasmid/pAAV-CAG-FLEX-rc+%5BJaws-KGC-GFP-ER2%5D+(Plasmid+%2384445)/pmc13623931-245-1-3
    Average 93 stars, based on 26 article reviews
    viral construct - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: REV-ERB in GABAergic neurons controls diurnal hepatic insulin sensitivity.
    Article Snippet: .. AAV-TRE-Flag-REV-ERB-α and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from E. Boyden), pMA3211 (Addgene 46879)33, and FUW-M2rtTA (Addgene 20342)34. ..

    Article Title: Rev-erb in GABAergic Neurons Controls Diurnal Hepatic Insulin Sensitivity
    Article Snippet: .. AAV-TRE-Flag-Rev-erbα and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from Edward Boyden), pMA3211 (Addgene 46879) , and FUW-M2rtTA (Addgene 20342) . ..

    Construct:

    Article Title: REV-ERB in GABAergic neurons controls diurnal hepatic insulin sensitivity.
    Article Snippet: .. AAV-TRE-Flag-REV-ERB-α and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from E. Boyden), pMA3211 (Addgene 46879)33, and FUW-M2rtTA (Addgene 20342)34. ..

    Article Title: Rev-erb in GABAergic Neurons Controls Diurnal Hepatic Insulin Sensitivity
    Article Snippet: .. AAV-TRE-Flag-Rev-erbα and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from Edward Boyden), pMA3211 (Addgene 46879) , and FUW-M2rtTA (Addgene 20342) . ..

    Plasmid Preparation:

    Article Title: REV-ERB in GABAergic neurons controls diurnal hepatic insulin sensitivity.
    Article Snippet: .. AAV-TRE-Flag-REV-ERB-α and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from E. Boyden), pMA3211 (Addgene 46879)33, and FUW-M2rtTA (Addgene 20342)34. ..

    Article Title: The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding
    Article Snippet: .. The EGFP ORF region of the pAAV-FLEX-GFP plasmid (Addgene plasmid #28304, a gift from Edward Boyden) was replaced by 3xFlag-mBecn1 F121A ORF using KpnI and XhoI restriction enzymes (NEB) and the In-Fusion HD Plus cloning kit. .. The AAV2/8 virus was produced by the Sanford Burnham Prebys Medical Discovery Institute Viral Vector Core Facility.

    Article Title: Rev-erb in GABAergic Neurons Controls Diurnal Hepatic Insulin Sensitivity
    Article Snippet: .. AAV-TRE-Flag-Rev-erbα and AAV-FLEX-rtTAM2 plasmids were constructed based on the pAAV-FLEX-GFP plasmid (Addgene 28304, from Edward Boyden), pMA3211 (Addgene 46879) , and FUW-M2rtTA (Addgene 20342) . ..

    Article Title: Excitatory Pathways from the Lateral Habenula Enable Propofol-Induced Sedation
    Article Snippet: .. pAAV-FLEX-GFP plasmid , Addgene , 28304. .. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA , Addgene , 20298.

    Cloning:

    Article Title: The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding
    Article Snippet: .. The EGFP ORF region of the pAAV-FLEX-GFP plasmid (Addgene plasmid #28304, a gift from Edward Boyden) was replaced by 3xFlag-mBecn1 F121A ORF using KpnI and XhoI restriction enzymes (NEB) and the In-Fusion HD Plus cloning kit. .. The AAV2/8 virus was produced by the Sanford Burnham Prebys Medical Discovery Institute Viral Vector Core Facility.



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